gcamp6f construct Search Results


gcamp6f expression construct  (StemCore Laboratories)
90
StemCore Laboratories gcamp6f expression construct
Gcamp6f Expression Construct, supplied by StemCore Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gcamp6f+construct/pm35468848-64-7-12?v=StemCore+Laboratories
Average 90 stars, based on 1 article reviews
gcamp6f expression construct - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
gcamp6f genetic construct  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics gcamp6f genetic construct
Spontaneous Neuronal Activity in the 3D In Vitro Biomimetic Cortical Culture (A) Representative fluorescence time-lapse image stacks of 3-week-old 3D cultures expressing <t>GCaMP6f.</t> Images are pseudo-colored to highlight Ca + + transients. Scale bar, 500 μm. (B) Mask with 37 hexagonal ROIs (dimensions: area = 0.22 mm 2 , side to side and center-to-center distances = 500 μm) is applied to the time-lapse image stack. (C) Extraction of time-series trace of average fluorescence intensity from the projection of 3D culture in each ROI. (D) Representative traces of spontaneous Ca + + transients from individual ROIs at 2 weeks, 3 weeks, and 3 weeks post-tetrodotoxin (TTX, 40 μM) treatment. Fluorescence changes are normalized to the basal fluorescence intensity (dF/F 0 ). (E) Percentage of all ROIs containing Ca + + event(s) (mean ± SEM). Each data point represents a unique 3D tissue culture sample. (F) Ca + + event frequency at 2 and 3 weeks (mean ± SEM). Each data point represents average frequency of non-zero event ROIs of a sample. For (E) and (F), n = 23 samples from 3 to 4 matched experiments for 2-week and 3-week samples. An unpaired t test was used for statistical significance. ∗∗p < 0.001. See also <xref ref-type=Figure S5 and . " width="250" height="auto" />
Gcamp6f Genetic Construct, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gcamp6f+construct/pmc07452433-59-13-9?v=BioMimetic+Therapeutics
Average 90 stars, based on 1 article reviews
gcamp6f genetic construct - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


Spontaneous Neuronal Activity in the 3D In Vitro Biomimetic Cortical Culture (A) Representative fluorescence time-lapse image stacks of 3-week-old 3D cultures expressing GCaMP6f. Images are pseudo-colored to highlight Ca + + transients. Scale bar, 500 μm. (B) Mask with 37 hexagonal ROIs (dimensions: area = 0.22 mm 2 , side to side and center-to-center distances = 500 μm) is applied to the time-lapse image stack. (C) Extraction of time-series trace of average fluorescence intensity from the projection of 3D culture in each ROI. (D) Representative traces of spontaneous Ca + + transients from individual ROIs at 2 weeks, 3 weeks, and 3 weeks post-tetrodotoxin (TTX, 40 μM) treatment. Fluorescence changes are normalized to the basal fluorescence intensity (dF/F 0 ). (E) Percentage of all ROIs containing Ca + + event(s) (mean ± SEM). Each data point represents a unique 3D tissue culture sample. (F) Ca + + event frequency at 2 and 3 weeks (mean ± SEM). Each data point represents average frequency of non-zero event ROIs of a sample. For (E) and (F), n = 23 samples from 3 to 4 matched experiments for 2-week and 3-week samples. An unpaired t test was used for statistical significance. ∗∗p < 0.001. See also <xref ref-type=Figure S5 and . " width="100%" height="100%">

Journal: iScience

Article Title: Functional Characterization of Three-Dimensional Cortical Cultures for In Vitro Modeling of Brain Networks

doi: 10.1016/j.isci.2020.101434

Figure Lengend Snippet: Spontaneous Neuronal Activity in the 3D In Vitro Biomimetic Cortical Culture (A) Representative fluorescence time-lapse image stacks of 3-week-old 3D cultures expressing GCaMP6f. Images are pseudo-colored to highlight Ca + + transients. Scale bar, 500 μm. (B) Mask with 37 hexagonal ROIs (dimensions: area = 0.22 mm 2 , side to side and center-to-center distances = 500 μm) is applied to the time-lapse image stack. (C) Extraction of time-series trace of average fluorescence intensity from the projection of 3D culture in each ROI. (D) Representative traces of spontaneous Ca + + transients from individual ROIs at 2 weeks, 3 weeks, and 3 weeks post-tetrodotoxin (TTX, 40 μM) treatment. Fluorescence changes are normalized to the basal fluorescence intensity (dF/F 0 ). (E) Percentage of all ROIs containing Ca + + event(s) (mean ± SEM). Each data point represents a unique 3D tissue culture sample. (F) Ca + + event frequency at 2 and 3 weeks (mean ± SEM). Each data point represents average frequency of non-zero event ROIs of a sample. For (E) and (F), n = 23 samples from 3 to 4 matched experiments for 2-week and 3-week samples. An unpaired t test was used for statistical significance. ∗∗p < 0.001. See also Figure S5 and .

Article Snippet: For functional analysis of network activity in our 3D biomimetic cortical culture, a GCaMP6f ( ) genetic construct was expressed in neurons for recording temporal calcium (Ca ++ ) concentration changes, called Ca ++ transients.

Techniques: Activity Assay, In Vitro, Fluorescence, Expressing, Extraction